DNA - FINGERPRINTING

DNA fingerprinting is also know as DNA profiling is a process of determining an individuals DNA characteristics. This is a forensic technique in criminal investigations, comparing criminal suspect's profiles to DNA to identify the like hood of their involvement in the crime. Also can be used for paternity tests. 99 % of human DNA sequences are the same in every person. Remaining sequences are different . It makes every individual unique.




This is used to distinguish and identify the individuals of a same species by using their DNA samples. These biological samples may be blood, hair, saliva, semen, body tissue cells. The DNA samples are isolated first. Then digested by restriction endonucleases. After this electrophoretic separation of fragments are done. These fragments are transferred into nylon or nitrocellulose membrane. This is followed by probing, hybridization and autoradiography. From this banding pattern is analyzed. 





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